As the population density of wild boars (Sus scrofa) increases worldwide, there is a concern that infectious pathogens may be transmitted to other animals including pigs. Reducing wild boar populations through hunting is being applied as a main way to prevent the disease transmission among animal species; however, hunting alone can not be an effective control method. Several countries have been using the gonadotropin-releasing hormone (GnRH) vaccine, which affects the release of reproductive hormones, as one of the solutions to control wildlife populations. We established the pestivirus based GnRH vaccine candidate that induces GnRH antibodies causes immunoneutralization and can also protect against swine infectious disease. To evaluate the immunogenicity of vaccine candidate, male pigs aged twenty weeks, were randomly allocated to each three groups: a negative control group and inoculated pig inoculated with an GnRH-viral vaccine candidate via intramuscular or oral administrations. Compared to control pigs, the GnRH virus-inoculated pigs not only showed delayed growth but relatively reduced sexual behaviors and aggressive activities. Correspondingly indirect ELISA method revealed that GnRH antibody levels tended to increase gradually in sera of GnRH vaccine candidate-inoculated pigs. Although the difference in testis weight was unclear, histopathological alterations such as the degenerated seminiferous tubules of testis and low sperm density of epididymis were identified in the GnRH vaccine candidate-inoculated pigs. It is expected that our GnRH vaccine candidate can be applied as potential vaccines that will broaden their applicability in eradicating pig disease and controlling wildlife populations.