Bovine herpesvirus 1 (BHV-1) poses a significant threat to the cattle industry, impacting both animal welfare and productivity. Although the rapid development of genome editing technologies has provided new tools for BHV-1 research, inadequate knowledge of host-virus interactions continues to limit the progress. To deepen the understanding, there is a need to for more effective implementation of genomic technologies. The objective of this study is to address the gap by investigating the interactions between BHV-1 and host cells from two directions. We aim to use fluorescence to label a non-essential but involved gene in the viral replication and two key exosome markers in the host cells. We will begin by inserting EGFP into virus genome with CRISPR/Cas9 technology, allowing us to trace the virus’s pathway without disrupting the viral replications. In parallel, we will label exosome markers to monitor changes in exosome secretion following viral infection in host cells. This dual approach will provide a comprehensive view of the pathways involved in viral replication and host responses, offering valuable insights into interactions between BHV-1 and host cells during the infection. With findings from this study, the industry will be able to develop more effective antiviral strategies and enhanced disease control measures to increase the resilience of cattle population to viral infections and reduce the impact of BHV-1 on the cattle industry.