The intracellular bacterium Wolbachia is a biocontrol tool used to limit the transmission of arboviruses such as dengue virus (DENV) by Aedes aegypti mosquitoes (1-3). While its antiviral properties in Ae. aegypti are well-established, the exact mechanisms by which Wolbachia inhibits viral replication remain unclear. Previous studies have used surrogate systems including Drosophila melanogaster-derived cells, and the alphavirus Sindbis virus to show that Wolbachia’s antiviral effects are confined to Wolbachia-infected cells and do not extend to neighbouring Wolbachia-free cells (4). Since the antiviral effects of Wolbachia can be host-specific, here we replicate these experiments using biologically-relevant Ae. aegypti-derived cells and DENV. By co-culturing cells with or without Wolbachia in a transwell system, we assessed whether Wolbachia-infected cells can restrict DENV replication in Wolbachia-free cells separated by a porous membrane. Our findings demonstrate that Wolbachia-free cells co-cultured with Wolbachia-infected cells had no reduction in viral replication compared to those co-cultured with other Wolbachia-free cells. This indicates that Ae. aegypti cells infected with Wolbachia do not release factors that can confer antiviral protection to surrounding Wolbachia-free cells and provides further insight into the mechanism of viral restriction by Wolbachia.